Wendling F, Moreau-Gachelin F, Tambourin P
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3614-8. doi: 10.1073/pnas.78.6.3614.
Appearance of tumorigenic cells was studied in DBA/2 and ICFW mice infected either with the polycythemia-inducing or the anemia-inducing strain of Friend leukemia virus. Tumorigenicity was defined by transplantability of virus-infected cells into the omentum of an isogeneic preirradiated host. Tumorigenic cells were detected in 50% of the leukemic donors 3 wk after infection by the polycythemia-inducing strain and 7-8 wk after infection by the anemia-inducing strain. These cells appeared first in the spleen and later in peripheral blood, bone marrow, and liver. They consisted of a heterogeneous population at different degrees of malignancy as determined by successive transfers in vivo and in vitro. The observations clearly show that leukemias induced by Friend viruses evolve by multistep processes, in which different stages of malignancy can be detected.
在感染了诱导红细胞增多或诱导贫血的弗瑞德白血病病毒株的DBA/2和ICFW小鼠中，研究了致瘤细胞的出现情况。致瘤性通过将病毒感染细胞移植到同基因预先照射的宿主的网膜中来定义。在感染诱导红细胞增多病毒株3周后，50%的白血病供体中检测到致瘤细胞；在感染诱导贫血病毒株7 - 8周后，也检测到了致瘤细胞。这些细胞首先出现在脾脏，随后出现在外周血、骨髓和肝脏中。通过体内和体外的连续传代确定，它们由不同恶性程度的异质群体组成。这些观察结果清楚地表明，弗瑞德病毒诱导的白血病是通过多步骤过程发展的，其中可以检测到不同的恶性阶段。
