Muscettola G, Potter W Z, Gordon E K, Goodwin F K
Psychiatry Res. 1981 Jun;4(3):267-76. doi: 10.1016/0165-1781(81)90028-7.
Reported mean values for urinary 3-methoxy-4-hydroxyphenylglycol (MHPG) for both depressed patients and controls vary substantially between laboratories. The present study describes methodological sources of variance affecting urinary MHPG values. Although intra-assay variance is small (coefficient of variation (CV) = 4.6%), the interassay variance is high (CV = 14.3%) and suggests caution in the interpretation of small group differences. The gas chromatographic (GC) method in this laboratory produces values highly correlated (r = 0.98) with those obtained by a reference gas chromatographic-mass spectrometric (GC-MS) method. The absolute values obtained by GC are 20% higher than those by GC-MS--a problem that may be shared to a varying extent by all GC assays.
methodological differences do contribute to some of the variation in reported values of urinary MHPG.
抑郁症患者和对照组尿中3-甲氧基-4-羟基苯乙二醇（MHPG）的报告均值在不同实验室之间差异很大。本研究描述了影响尿中MHPG值的方法学差异来源。尽管批内差异较小（变异系数（CV）=4.6%），但批间差异较大（CV = 14.3%），这表明在解释小组差异时需谨慎。本实验室的气相色谱（GC）法所得值与参考气相色谱-质谱联用（GC-MS）法所得值高度相关（r = 0.98）。GC法获得的绝对值比GC-MS法高20%——这一问题可能在不同程度上存在于所有GC检测中。
方法学差异确实导致了尿中MHPG报告值的部分差异。
