Tosi R, Tanigaki N, Sorrentino R, Accolla R, Corte G
Eur J Immunol. 1981 Sep;11(9):721-6. doi: 10.1002/eji.1830110910.
A monoclonal anti-human Ia antibody, PTF 29, was tested for its ability to bind purified, 125I-labeled human Ia preparations. It was found that the binding level increases considerably in the presence of a second monoclonal antibody. Experimental conditions were selected under which only the binding of PTF 29 and not the binding of the second antibody could be determined. Under these conditions, it was found that "helped" PTF 29 binding has higher affinity and is exerted on a molecular subset different from that bound by PTF 29 alone. This phenomenon, while not easily accommodated in the present conceptual framework of the human Ia system, appears in itself interesting and may have more general implications.
一种单克隆抗人Ia抗体PTF 29，被检测其结合纯化的、125I标记的人Ia制剂的能力。发现当存在第二种单克隆抗体时，结合水平显著增加。选择了仅能测定PTF 29的结合而不能测定第二种抗体结合的实验条件。在这些条件下，发现“辅助的”PTF 29结合具有更高的亲和力，并且作用于与单独的PTF 29所结合的分子亚群不同的分子亚群。这种现象虽然难以纳入目前人类Ia系统的概念框架，但本身似乎很有趣，可能具有更广泛的意义。
