Quinn C A, Goodfellow P N, Povey S, Walsh F S
Proc Natl Acad Sci U S A. 1981 Aug;78(8):5031-5. doi: 10.1073/pnas.78.8.5031.
Somatic cell hybrids have been prepared at high frequency between the rat muscle cell line L6 and human fetal muscle cells. The hybrid cells express several human gene products including an antigen, 12E7, controlled by the human X chromosome, Thy-1, and several human isoenzymes. In addition, one clone (37-11) expresses a human muscle-specific surface antigen (5.1 H11) and, under appropriate conditions, can be induced to form myotubes. Upon myotube formation, this clone switches on the production of the human muscle-specific creatine kinase (ATP:creatine N-phosphotransferase, EC 2.7.3.2) M subunit. This is an example of developmental regulation of human muscle-specific genes in somatic cell hybrids.
已经在大鼠肌肉细胞系L6和人胎儿肌肉细胞之间高频制备了体细胞杂种。杂种细胞表达几种人类基因产物，包括一种由人类X染色体控制的抗原12E7、Thy-1和几种人类同工酶。此外，一个克隆（37-11）表达一种人类肌肉特异性表面抗原（5.1 H11），并且在适当条件下可以被诱导形成肌管。在肌管形成时，这个克隆开启人类肌肉特异性肌酸激酶（ATP：肌酸N-磷酸转移酶，EC 2.7.3.2）M亚基的产生。这是体细胞杂种中人类肌肉特异性基因发育调控的一个例子。
