Walter R J, Berns M W
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6927-31. doi: 10.1073/pnas.78.11.6927.
Digital processing techniques can be used to greatly enhance the available information in an optical image. Although this technology has been routinely used in many fields for a number of years, little application of digital image-processing techniques have been made toward analysis and enhancement of the types of images seen most often by the research biologist. We describe here a computer-based video microscope system that is capable of performing extensive manipulation and enhancement of microscope images in real time. The types of manipulations possible with these techniques greatly surpass the enhancement capabilities of photographic or video techniques alone. The speed and flexibility of this system enables experimental manipulation of the microscopic specimen based on its live processed image. These features greatly extend the power and versatility of the light microscope.
数字处理技术可用于大幅增强光学图像中的可用信息。尽管这项技术多年来已在许多领域得到常规应用，但数字图像处理技术在分析和增强研究生物学家最常看到的图像类型方面的应用却很少。我们在此描述一种基于计算机的视频显微镜系统，它能够实时对显微镜图像进行广泛的处理和增强。这些技术所能实现的处理类型大大超越了仅靠摄影或视频技术的增强能力。该系统的速度和灵活性使得能够根据实时处理后的图像对微观标本进行实验操作。这些特性极大地扩展了光学显微镜的功能和通用性。
