Dunn R F, O'Leary D P, Kumley W E
J Microsc. 1975 Nov;105(2):205-13. doi: 10.1111/j.1365-2818.1975.tb04051.x.
A computer-aided graphical analysis system is presented which can be used to quantify several types of information from micrographs of cross-sectioned peripheral nerves. A simple input consisting of eight points, corresponding to two diameters of each axon and two for each axon plus myelin, is used to compute statistical and numerical estimates of these diameters, the myelin thickness being expressed as a function of axon diameter, and both the spatial position and distribution of nerve fibres within the bundle. The computer programs presented and summarized here describe the procedure for digitizing the input data and the subsequent computations for editing and analysis. The general applicability of this approach for automated analyses of other anatomical areas is also presented.
本文介绍了一种计算机辅助图形分析系统，该系统可用于量化从横切外周神经显微照片中获取的几种类型的信息。通过一个简单的输入，即由八个点组成，分别对应每个轴突的两个直径以及每个轴突加髓鞘的两个直径，来计算这些直径的统计和数值估计，髓鞘厚度表示为轴突直径的函数，同时还能计算神经纤维束内的空间位置和分布。本文展示并总结的计算机程序描述了输入数据数字化的过程以及后续编辑和分析的计算方法。还介绍了这种方法在其他解剖区域自动分析中的普遍适用性。
