Nielson D W, Goerke J, Clements J A
Proc Natl Acad Sci U S A. 1981 Nov;78(11):7119-23. doi: 10.1073/pnas.78.11.7119.
We measured the pH of the alveolar subphase fluid by puncturing the most superficial alveoli of the exposed lungs of anesthetized rabbits with H+-selective and nonselective KCl microelectrodes. In these experiments, we bathed the lung surface with paraffin oil or buffered Ringer's solutions that had a CO2 tension of 40 Torr (1 Torr = 133.3 Pa) and found an alveolar pH of 6.92 +/- 0.01 (mean +/- sEM). When the pH of the surface buffer was below 6.7 or above 7.5, alveolar pH varied with surface buffer pH. With the nonselective electrode, we did not find a significant electrical potential difference between the alveolar fluid and the pleural surface. These results are consistent with active transport of H+ (or HCO3(-) across alveolar epithelium.
我们通过用氢离子选择性和非选择性氯化钾微电极穿刺麻醉兔暴露肺的最表层肺泡来测量肺泡亚相液体的pH值。在这些实验中，我们用石蜡油或二氧化碳张力为40托（1托 = 133.3帕）的缓冲林格氏液冲洗肺表面，发现肺泡pH值为6.92±0.01（平均值±标准误）。当表面缓冲液的pH值低于6.7或高于7.5时，肺泡pH值随表面缓冲液pH值而变化。使用非选择性电极时，我们未发现肺泡液与胸膜表面之间存在显著的电位差。这些结果与氢离子（或碳酸氢根离子）跨肺泡上皮的主动转运一致。
