Sakuma T, Suzuki S, Usuda K, Handa M, Okaniwa G, Nakada T, Fujimura S, Matthay M A
Department of Surgery, Sendai Kosei Hospital, Tohoku University, Japan.
J Appl Physiol (1985). 1996 May;80(5):1681-6. doi: 10.1152/jappl.1996.80.5.1681.
Although hypothermia abolishes alveolar fluid clearance in the in situ goat lung and in the ex vivo human lung, it is unknown whether alveolar fluid clearance resumes in lungs that are rewarmed after severe hypothermia. An isosmolar albumin solution was instilled into resected human lungs that were rewarmed to 37 degrees C after hypothermia (7 +/- 3 degrees C), and then alveolar fluid clearance was measured by the concentration of albumin in the alveolar fluid sample after 4 h. In control experiments in lungs that had not been cooled and rewarmed, alveolar fluid clearance was 11 +/- 2% over 4 h. In separate experiments, hypothermia completely abolished alveolar fluid clearance. However, alveolar fluid clearance resumed to a normal level of 12 +/- 1% over 4 h in the lungs that were rewarmed after hypothermia. Amiloride decreased alveolar fluid clearance by 47% in the rewarmed lungs. Terbutaline increased alveolar fluid clearance by nearly 300% in 2-h experiments in the rewarmed lungs (P < 0.05). The results of this study indicate that alveolar sodium-channel transport mechanisms are preserved in resected human lungs that are exposed to rewarming after hypothermia.
虽然低温会消除原位山羊肺和离体人肺中的肺泡液体清除功能，但严重低温后复温的肺中肺泡液体清除功能是否恢复尚不清楚。将等渗白蛋白溶液注入低温（7±3℃）后复温至37℃的离体人肺中，然后在4小时后通过肺泡液样本中白蛋白的浓度测量肺泡液体清除率。在未冷却和复温的肺的对照实验中，4小时内肺泡液体清除率为11±2%。在单独的实验中，低温完全消除了肺泡液体清除功能。然而，低温后复温的肺中，4小时内肺泡液体清除率恢复到正常水平12±1%。氨氯地平使复温肺中的肺泡液体清除率降低了47%。在复温肺的2小时实验中，特布他林使肺泡液体清除率提高了近300%（P<0.05）。本研究结果表明，在低温后复温的离体人肺中，肺泡钠通道转运机制得以保留。
