Winkel P, Olesen T
J Histochem Cytochem. 1981 Dec;29(12):1382-6. doi: 10.1177/29.12.6948039.
An alarm system for the detection of abnormal leukocytes using off-line data processing of the image of the peroxidase channel oscilloscope picture of the Hemalog-D is presented. The basic idea is that areas on the oscilloscope picture where more than a negligible number of nonpathological leukocytes may be depicted are delimited from the remaining area, which is divided into three alarm zones. The corresponding alarm quantities are the large unstained cells (LUC) or the unstained alarm (UA), the intermediate alarm (IA), and the stained alarm (SA). Reference intervals for these alarms were established using blood specimens from 15 healthy subjects. The system was tested using blood specimens from four patients with acute lymphoblastic leukemia (ALL) and 11 patients with acute myeloblastic leukemia (AML). The UA was the best alarm overall, but for seven of the AML specimens the IA or the SA alarm was superior. The high peroxidase (HPX) and the remainder alarms were inferior to the other alarms. Using the reference mean plus two standard deviations as a cutoff value, the smallest blast cell number fraction detectable by the best alarm was calculated to be smaller than 2% for each of the AML specimens, while it ranged from 7 to 2.9% for the ALL specimens.
介绍了一种利用Hemalog-D过氧化物酶通道示波器图像的离线数据处理来检测异常白细胞的报警系统。其基本思想是，在示波器图像上，将可能描绘出数量超过可忽略不计的非病理性白细胞的区域与其余区域区分开来，其余区域又分为三个报警区。相应的报警量为大未染色细胞（LUC）或未染色报警（UA）、中间报警（IA）和染色报警（SA）。使用15名健康受试者的血液标本建立了这些报警的参考区间。该系统使用4例急性淋巴细胞白血病（ALL）患者和11例急性髓细胞白血病（AML）患者的血液标本进行了测试。总体而言，UA是最佳报警，但对于7份AML标本，IA或SA报警更优。高过氧化物酶（HPX）和其余报警比其他报警差。以参考均值加两个标准差作为临界值，计算出最佳报警可检测到的最小原始细胞数分数，对于每份AML标本均小于2%，而对于ALL标本则在7%至2.9%之间。
