Schmid S L, Matsumoto A K, Rothman J E
Proc Natl Acad Sci U S A. 1982 Jan;79(1):91-5. doi: 10.1073/pnas.79.1.91.
Triskelions, trimeric complexes of clathrin and associated light chains, are the assembly units of clathrin coats [Ungewickell, E. & Branton, D. (1981) Nature (London) 289, 420-422; Kirchhausen, T. & Harrison, S. C. (1981) Cell 23, 755-761]. We report here that triskelions whose outer arms have been removed by trypsin digestion retain the ability to be assembled into coats. These digested timers contain a 110,000 molecular weight domain of clathrin and lack intact light chains.
三脚蛋白复合体是网格蛋白与相关轻链的三聚体复合物,是网格蛋白包被的组装单位[翁格维克尔,E. & 布兰顿,D.(1981年)《自然》(伦敦)289卷,420 - 422页;基尔豪森,T. & 哈里森,S. C.(1981年)《细胞》23卷,755 - 761页]。我们在此报告,经胰蛋白酶消化去除外臂的三脚蛋白复合体仍保留组装成包被的能力。这些消化后的三聚体包含一个分子量为110,000的网格蛋白结构域,且缺乏完整的轻链。