Brzeziński W, Brozyński K, Kmieć B, Fortak W
Folia Histochem Cytochem (Krakow). 1981;19(3):149-60.
The interscapular brown adipose tissue in male, white Wistar rats in the 1,3,6,15,30, and 60th day of regeneration of adrenals after their enucleation weas investigated. The tissue slides were stained with H and E, with Masson's triple stain and with Sudan B. The activity of the following dehydrogenses was evaluated: Succinic dehydrogenase, delta 5,3 beta-hydroxysteroid dehydrogenase and the activity of the following enzymes; monoaminooxidase (MAO), Alkaline phosphatase (AlPh) and acid phosphatase (AcPh) was studied. Between the first ad 30th day of regeneration of adrenals morphochemical changes were observed; a great number of cells with eosinophylic cytoplasm appeared and with minute vacuols in their cytoplasm, the blood vessels became more numerous and enlarged and the activity of the enzymes tested was enhanced, instead sudanophilia was weaken and the reaction of corticosteriods significantly intensified. The changes observed may indicate on an enhanced hormonal activity. The brown adipose tissue may function as an endocrine gland which supports the regenerating adrenals of the white rat, enables survival and preservation of homeostasis during the insufficiency of the adrenal cortex after adrenal enucleation. The initiation of the endocrine activity of the brown adipose tissue in the course of regeneration of the adrenals proceeds probably without the direct participation of catecholamines originating from the adrenals.
对摘除肾上腺后处于再生第1、3、6、15、30和60天的雄性白色Wistar大鼠的肩胛间棕色脂肪组织进行了研究。组织切片用苏木精和伊红染色、用马森三色染色法以及苏丹B染色。评估了以下脱氢酶的活性：琥珀酸脱氢酶、δ5,3β - 羟基类固醇脱氢酶，并研究了以下酶的活性：单胺氧化酶（MAO）、碱性磷酸酶（AlPh）和酸性磷酸酶（AcPh）。在肾上腺再生的第1天至第30天之间观察到形态化学变化；出现了大量细胞质嗜酸性且细胞质中有微小液泡的细胞，血管变得更多且更粗大，所测试酶的活性增强，相反嗜苏丹性减弱，皮质类固醇反应显著增强。观察到的这些变化可能表明激素活性增强。棕色脂肪组织可能作为一种内分泌腺发挥作用，支持白色大鼠再生的肾上腺，在肾上腺摘除后肾上腺皮质功能不全期间维持生存并保持体内平衡。棕色脂肪组织内分泌活性的启动在肾上腺再生过程中可能在没有肾上腺来源的儿茶酚胺直接参与的情况下进行。
