Löwenberg B, Hagemeijer A, Swart K
Blood. 1982 Mar;59(3):641-5.
Bone marrow and blood cells of a patient with acute monoblastic leukemia with subclones marked with specific karyotypic abnormalities were investigated. In order to more precisely evaluate the proliferative abilities of these populations, leukemic cell enriched fractions were prepared and incubated in two colony assays. Colony forming cells of the disparate clones had growth advantages in different systems which shows that their proliferation depended on the presence of selective stimulatory factors in culture. In one assay, at diagnosis, colonies from the minor clone were demonstrated exclusively. It is suggested that the assays measured distinct cellular stages of myeloid differentiation and the findings indicate that prior to diagnosis the neoplasm had evolved into subsets with progressive dedifferentiation. Differences of growth in vitro correlated with the different roles of these clones in the clinical history of the disease. Approaches based on differential cloning of tumor stem cells as in this example, may be useful for discriminating biological properties of heterogeneous subpopulations within neoplasms, and may facilitate the cytogenetic recognition of minimal clones among composite malignant cell specimens.
对一名患有急性单核细胞白血病且带有特定核型异常标记亚克隆的患者的骨髓和血细胞进行了研究。为了更精确地评估这些细胞群体的增殖能力，制备了富含白血病细胞的组分，并在两种集落测定中进行培养。不同克隆的集落形成细胞在不同系统中具有生长优势，这表明它们的增殖依赖于培养中选择性刺激因子的存在。在一项测定中，在诊断时，仅显示出来自次要克隆的集落。提示这些测定测量了髓系分化的不同细胞阶段，并且结果表明在诊断之前肿瘤已演变为具有进行性去分化的亚群。体外生长的差异与这些克隆在疾病临床过程中的不同作用相关。如本例中基于肿瘤干细胞差异克隆的方法，可能有助于区分肿瘤内异质亚群的生物学特性，并可能促进复合恶性细胞标本中最小克隆的细胞遗传学识别。
