Sablina O V, Dymshits G M
Tsitologiia. 1978 Jul;20(7):764-9.
In connection with a supposition concerning the role of nucleases in the production of chromosome breaks in the herpes simplex virus-infected cells, the stability of DNA of the infected cultured cells was studied. It was established that the infection of cells with herpes simplex virus resulted in a partial degradation of cellular DNA of permissive and non-permissive cells. In human embryo fibroblast culture products of hydrolysis of cellular DNA may be reutilized by the synthetising virus. In cells of clone M15, in which no mature virus is produced, products of hydrolysis of DNA are revealed in acid-soluble pool. The observed degradation of DNA apparently is not associated with the cell death. It cannot be excluded that a partial degradation of DNA in the cell due to viral infection may bring about a production of visible chromosome breaks.
关于核酸酶在单纯疱疹病毒感染细胞中产生染色体断裂作用的一种假设，对感染培养细胞的DNA稳定性进行了研究。已确定单纯疱疹病毒感染细胞会导致允许性和非允许性细胞的细胞DNA部分降解。在人胚成纤维细胞培养物中，细胞DNA水解产物可被正在合成的病毒重新利用。在不产生成熟病毒的克隆M15细胞中，DNA水解产物出现在酸溶性组分中。观察到的DNA降解显然与细胞死亡无关。不能排除病毒感染导致细胞内DNA部分降解可能引起可见染色体断裂的产生。
