Platica M, Bojko C, Clincea R, Hollander V
J Natl Cancer Inst. 1982 Feb;68(2):325-8.
The role for lipopolysaccharide (LPS) and a pristane-induced peritoneal exudate (PIPE) on the in vitro development of murine plasmacytoma was studied. MOPC-315 cell suspensions showed little tendency for colony formation in a soft agar culture medium. Additions of LPS and PIPE were required for maximal colony formation. The LPS effect was dose-dependent down to nanogram quantities. PIPE prepared from inbred strains of mice not susceptible to pristane-induced plasmacytoma (DBA/2) or from normal peritoneal washings was ineffective. PIPE activity was radioresistant and not transferable by cell-free conditioned medium. Three strains of transplantable plasmacytomas showed colony formation stimulation by LPS plus PIPE, but LPS and PIPE were ineffective with lymphosarcoma P1798.
研究了脂多糖（LPS）和 pristane 诱导的腹腔渗出液（PIPE）对小鼠浆细胞瘤体外发育的作用。MOPC - 315 细胞悬液在软琼脂培养基中形成集落的倾向很小。需要添加 LPS 和 PIPE 才能实现最大程度的集落形成。LPS 的作用在纳克量以下呈剂量依赖性。从不易患 pristane 诱导的浆细胞瘤的近交系小鼠（DBA/2）制备的 PIPE 或正常腹腔冲洗液均无效。PIPE 活性具有放射抗性，且不能通过无细胞条件培养基转移。三株可移植的浆细胞瘤显示 LPS 加 PIPE 可刺激集落形成，但 LPS 和 PIPE 对淋巴肉瘤 P1798 无效。
