Endrich B, Hammersen F, Götz A, Messmer K
J Natl Cancer Inst. 1982 Mar;68(3):475-85.
Transparent chambers and permanent indwelling catheters were implanted in the dorsal skin flaps of 24 inbred golden Syrian hamsters. After 48 hours, 4 x 10(4) amelanotic melanoma cells (A-Mel-3) were transplanted sc (12 hamsters) in areas exposed for intravital microscopy. A platinum multiwire electrode and techniques for a quantitative analysis of television images were used for measurements of local oxygen pressure (PO2), capillary blood cell velocity, capillary density, and capillary diameter and length, whereas capillary morphology was evaluated by electron microscopy. Compared to the mean local PO2 of the skin flaps of controls (12 hamsters), the mean local PO2 on the tumor surface of melanoma-bearing hamsters decreased with tumor size. Although capillary density of the melanoma was elevated 4 days after tumor transplantation, it decreased significantly until day 12. Of hemodynamic significance were huge platelet conglomerates in short, dilated capillaries. Electron microscopy revealed endothelial hyperplasia, open endothelial junctions, and disintegration of the capillary endothelium. These findings strongly suggest that elevated microvascular resistance, intratumor tissue pressure, and widening of intercapillary distances in the melanoma might significantly diminish the impact of chemotherapeutic treatment.
将透明小室和永久性留置导管植入24只近交系金黄叙利亚仓鼠的背部皮瓣中。48小时后，将4×10⁴个无黑色素黑色素瘤细胞（A-Mel-3）皮下移植到12只仓鼠用于活体显微镜观察的暴露区域。使用铂多丝电极和电视图像定量分析技术测量局部氧分压（PO₂）、毛细血管血细胞流速、毛细血管密度、毛细血管直径和长度，而毛细血管形态则通过电子显微镜进行评估。与对照组（12只仓鼠）皮瓣的平均局部PO₂相比，携带黑色素瘤仓鼠肿瘤表面的平均局部PO₂随肿瘤大小而降低。尽管肿瘤移植后4天黑色素瘤的毛细血管密度升高，但到第12天显著下降。具有血流动力学意义的是短而扩张的毛细血管内有巨大的血小板聚集体。电子显微镜显示内皮细胞增生、内皮连接开放和毛细血管内皮崩解。这些发现强烈表明，黑色素瘤中微血管阻力升高、肿瘤内组织压力增加以及毛细血管间距增宽可能会显著削弱化疗治疗效果。
