Entenberg David, Kedrin Dmitriy, Wyckoff Jeffrey, Sahai Erik, Condeelis John, Segall Jeffrey E
Albert Einstein College of Medicine, Bronx, New York.
These authors contributed equally to this work.
Curr Protoc Cell Biol. 2013 Mar;Chapter 19:19.7.1-19.7.19. doi: 10.1002/0471143030.cb1907s58.
This unit describes the methods that we have been developing for analyzing tumor cell motility in mouse and rat models of breast cancer metastasis. Rodents are commonly used both to provide a mammalian system for studying human tumor cells (as xenografts in immunocompromised mice) as well as for following the development of tumors from a specific tissue type in transgenic lines. The Basic Protocol in this unit describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included.
本单元介绍了我们一直在开发的用于分析乳腺癌转移小鼠和大鼠模型中肿瘤细胞运动性的方法。啮齿动物通常既用于提供一个研究人类肿瘤细胞的哺乳动物系统（作为免疫缺陷小鼠中的异种移植），也用于跟踪转基因品系中特定组织类型肿瘤的发展。本单元的基本方案描述了用于生成乳腺肿瘤并对其进行成像的标准方法。还包括标记巨噬细胞、血管成像和图像分析的其他方案。
