Hayashi J I, Tagashira Y, Moriwaki K, Yosida T H
Mol Gen Genet. 1981;184(3):337-41. doi: 10.1007/BF00352501.
Extensive polymorphism was found in mitochondrial DNAs (mtDNAs) of Norway rats (Rattus norvegicus). The restriction endonuclease cleavage patterns of mtDNAs of laboratory rats, wild rats, tumor cells, and culture cells were compared. The polymorphism is defined by two criteria; one is cleavage site variation and the other is length polymorphism of restriction fragments. The cleavage site variation may be caused by point mutation, and the length polymorphism by sequence deletions or insertions. At least five types, types A-E, were identified by cleavage site variations, and two groups, a and b, were identified by length polymorphism of one HpaII fragment, Hpa5. All types except type C belonged to either group-a or group-b, whereas both groups were found in type C. Differentiation of polymorphic Norway rat mtDNA types and the experimental use of the polymorphism are discussed.
在褐家鼠（Rattus norvegicus）的线粒体DNA（mtDNA）中发现了广泛的多态性。对实验大鼠、野生大鼠、肿瘤细胞和培养细胞的mtDNA的限制性内切酶切割模式进行了比较。这种多态性由两个标准定义；一个是切割位点变异，另一个是限制性片段的长度多态性。切割位点变异可能由点突变引起，长度多态性由序列缺失或插入引起。通过切割位点变异鉴定出至少五种类型，即A - E型，通过一个HpaII片段Hpa5的长度多态性鉴定出两组，即a组和b组。除C型外的所有类型都属于a组或b组，而C型中同时发现了这两组。讨论了多态性褐家鼠mtDNA类型的分化以及该多态性的实验用途。
