Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität, Goethestrasse 33, D-8000, München 2, Germany.
Curr Genet. 1979 Dec;1(1):13-9. doi: 10.1007/BF00413303.
A restriction endonuclease cleavage map of rat liver mitochondrial DNA is presented for the following enzymes: Xba I, Bgl II, Hae II, Bam HI, Hpa I, Hha I, Bcl I, Hind II, Hind III, Eco RI, Hpa II, Hae III, and Sau 3A. It was derived from complete and partial digestions with these enzymes, double digestions, and redigestions of defined fragments obtained with one enzyme with other restriction enzymes. By the use of these and further enzymes (Taq YI, Alu I) the mitochondrial DNA (ca. 15.6 Kb) can be dissected into a large number of defined fragments.
给出了大鼠肝线粒体 DNA 的限制内切酶酶切图谱，所用的酶有：Xba I、Bgl II、Hae II、Bam HI、Hpa I、Hha I、Bcl I、Hind II、Hind III、Eco RI、Hpa II、Hae III 和 Sau 3A。图谱由完全酶切、部分酶切、双酶切、单酶切后再用其它酶重切、及用部分酶切得到的特定片段进一步酶切得到。利用这些酶（Taq YI、Alu I）可以将线粒体 DNA（约 15.6 Kb）切割成大量特定片段。
