Parnham M J, Shoshan S, Schoenmaker H, Bonta I L
Prostaglandins. 1982 Jan;23(1):85-98. doi: 10.1016/0090-6980(82)90025-9.
Local administration of PGE2 (2 micrograms) to polyether sponges, implanted s.c. in rats, inhibited hydroxyproline and total protein accumulation, without altering relative amounts of collagen, when administered early during granuloma development. In contrast, while DNA as well as total protein accumulation was inhibited by local PGE2 treatment of established granuloma, hydroxyproline accumulation and the relative amounts of collagen were enhanced. This PGE2-induced collagen enhancement was associated with an increased type III : type I collagen ratio, possibly due to differential intracellular breakdown of newly synthesized collagen. The solubility of granuloma collagen was unaffected by PGE2. Impregnation of sponges with carrageenan before implantation, thereby giving macrophage-dominated granuloma, did not affect the changes in protein and DNA induced by later treatment with PGE2, but did reverse the PGE2-induced accumulation of hydroxyproline. This latter effect probably reflects macrophage-mediated, PGE2 enhancement of collagenolytic activity.
在大鼠皮下植入的聚醚海绵局部给予前列腺素E2（2微克），在肉芽肿形成早期给药时，可抑制羟脯氨酸和总蛋白积累，而不改变胶原蛋白的相对含量。相比之下，虽然局部用前列腺素E2处理已形成的肉芽肿可抑制DNA以及总蛋白积累，但羟脯氨酸积累和胶原蛋白的相对含量却增加。这种前列腺素E2诱导的胶原蛋白增加与III型：I型胶原蛋白比例增加有关，这可能是由于新合成的胶原蛋白在细胞内的差异性分解所致。肉芽肿胶原蛋白的溶解性不受前列腺素E2影响。在植入前用角叉菜胶浸渍海绵，从而形成以巨噬细胞为主的肉芽肿，这并不影响后期用前列腺素E2处理所诱导的蛋白质和DNA变化，但确实逆转了前列腺素E2诱导的羟脯氨酸积累。后一种效应可能反映了巨噬细胞介导的、前列腺素E2对胶原olytic活性的增强作用。
