Yoshima H, Shiraishi N, Matsumoto A, Maeda S, Sugiyama T, Kobata A
J Biochem. 1982 Jan;91(1):233-46. doi: 10.1093/oxfordjournals.jbchem.a133680.
The paper electrophoretic pattern of the oligosaccharides released from the plasma membranes of K-562 cells by hydrazinolysis was quite different from that of human erythrocyte membranes. Bio-Gel P-4 column chromatography in combination with sequential exoglycosidase digestion of the neutral oligosaccharide fractions revealed that all those from K-562 cells are of the high mannose type, while those from erythrocytes are of large complex type structures. Studies of the acidic oligosaccharides indicated that none of those obtained from K-562 cells contained the beta-N-acetylglucosamine residue linked at the C-4 position of the beta-mannosyl residue of the trimannosyl core, which occurs in most of the asparagine-linked sugar chains of human erythrocytes. This indicates that the glucosaminyltransferase that forms the GlcNAc beta 1 leads to 4Man beta 1 leads to group has not been expressed in K-562 cells.
通过肼解从K-562细胞膜释放的寡糖的纸电泳图谱与人类红细胞膜的图谱有很大不同。Bio-Gel P-4柱色谱结合中性寡糖馏分的顺序外切糖苷酶消化显示，来自K-562细胞的所有寡糖都是高甘露糖型，而来自红细胞的寡糖具有大的复合型结构。对酸性寡糖的研究表明，从K-562细胞获得的寡糖中没有一种含有连接在三甘露糖核心β-甘露糖基残基C-4位置的β-N-乙酰葡糖胺残基，而这种残基存在于人类红细胞的大多数天冬酰胺连接糖链中。这表明在K-562细胞中未表达形成GlcNAcβ1→4Manβ1→基团的葡糖胺基转移酶。
