Habeeb A F, Preston A M, Sanabria P, Escobales N
J Natl Cancer Inst. 1982 Apr;68(4):651-6.
Inbred male F344 rats treated with 20 mg methylazoxymethanol (MAM)/kg body weight showed minimal damage to DNA in bone marrow, spleen, and thymus and no suppression of the humoral immune response to human serum albumin (HSA). At high doses of MAM (40, 60, and 80 mg/kg), damage to DNA of bone marrow, spleen, and thymus was noted. The effect on the humoral immune responses was nonuniform. Some rats responded normally by eliciting precipitating antibodies, whereas others responded by synthesizing nonprecipitating antibodies at low levels. The production of nonprecipitating antibodies may indicate a restriction of the recognition of HSA antigenic determinants in rats given injections of MAM prior to immunization. The restriction may be a consequence of suppression or elimination of certain clones of lymphocytes responsible for recognition of certain antigenic determinants. This interference in some clones of lymphocytes would result in the synthesis of antibodies against one or two antigenic sites on the HSA and the concomitant inability of the produced antibodies to form complete lattice structure with antigen.
用20毫克甲基氧化偶氮甲醇（MAM）/千克体重处理的近交系雄性F344大鼠，其骨髓、脾脏和胸腺中的DNA损伤最小，且对人血清白蛋白（HSA）的体液免疫反应未受抑制。在高剂量的MAM（40、60和80毫克/千克）处理下，观察到骨髓、脾脏和胸腺的DNA受到损伤。对体液免疫反应的影响并不一致。一些大鼠通过产生沉淀抗体正常反应，而另一些大鼠则以低水平合成非沉淀抗体来反应。非沉淀抗体的产生可能表明在免疫前注射MAM的大鼠中，对HSA抗原决定簇的识别受到限制。这种限制可能是负责识别某些抗原决定簇的某些淋巴细胞克隆受到抑制或消除的结果。对某些淋巴细胞克隆的这种干扰将导致针对HSA上一个或两个抗原位点的抗体合成，以及所产生的抗体无法与抗原形成完整晶格结构。
