Varani J, Lovett E J
J Natl Cancer Inst. 1982 Jun;68(6):957-62.
Murine fibrosarcoma cells that vary in their transplantability in syngeneic mice were isolated from a heterogeneous parent tumor (induced in a female C57BL/6 inbred mouse) and maintained in culture by serial passage. Several biologic properties that discriminate between the high- and low-transplantable lines (including adhesiveness, motility, and levels of chymotrypsin-like esterase activity), as well as properties that do not separate these lines (e.g., in vitro growth rates and levels of protease and glycosidase activities), were measured at periodic intervals over 2 years. Ability to induce primary tumors and to induce metastases from these tumors were evaluated at the same intervals. The high- and low-transplantable lines were also transplanted into syngeneic mice at tumorigenic doses. Isolates from primary and metastatic tumors induced in the animals were reestablished in culture and examined for the various characteristics of passages 5, 10, 20, and 30. The properties of the cells maintained continually in culture remained stable throughout the 2-year observation period. Tumor isolates showed some evidence of modulation immediately after reestablishment in culture, but by passage 10 they appeared to be identical to the prototype parent lines. These data show that the fibrosarcoma cells do not undergo continual phenotypic "drift" as has been suggested to occur with other tumor lines maintained by serial passage in animals or in culture.
从一个异质性亲本肿瘤（在一只雌性C57BL/6近交小鼠中诱发）中分离出在同基因小鼠中移植能力各异的鼠纤维肉瘤细胞，并通过连续传代在培养中维持。在两年的时间里定期测量了几种区分高移植性和低移植性细胞系的生物学特性（包括黏附性、运动性和类胰凝乳蛋白酶酯酶活性水平），以及不能区分这些细胞系的特性（例如体外生长速率和蛋白酶及糖苷酶活性水平）。以相同的间隔评估诱导原发性肿瘤以及诱导这些肿瘤发生转移的能力。还将高移植性和低移植性细胞系以致瘤剂量移植到同基因小鼠中。将在动物中诱导产生的原发性和转移性肿瘤的分离物重新在培养中建立，并检查传代5、10、20和30时的各种特征。在整个两年的观察期内，持续在培养中维持的细胞特性保持稳定。肿瘤分离物在重新建立培养后立即显示出一些调节的迹象，但到传代10时，它们似乎与原型亲本细胞系相同。这些数据表明，纤维肉瘤细胞不会像在动物或培养中通过连续传代维持的其他肿瘤细胞系那样发生持续的表型“漂移”。
