Varani J, Grimstad I A, Knibbs R N, Hovig T, McCoy J P
Clin Exp Metastasis. 1985 Jan-Mar;3(1):45-59. doi: 10.1007/BF01758953.
Highly malignant cell lines and low-malignant cell lines isolated from three different methylcholanthrene-induced murine fibrosarcomas were examined for their ability to attach to plastic dishes and collagen-coated dishes under serum-free conditions and in the presence of serum. Most of the cells from the three highly malignant lines attached and spread under all conditions. By 72 h, there was a significant increase in the number of cells indicating that at least some of the cells had undergone division (even in the absence of serum). In contrast, fewer of the cells from the three low-malignant lines attached and spread on the plastic or collagen substrates in the absence of serum or in the presence of 0.1 per cent serum. However, when 15 micrograms laminin per dish was added along with the low-malignant cells, they then attached and spread on the plastic and collagen-coated dishes. Previous studies have indicated that the highly malignant lines express cell surface antigens that cross-react with laminin while the low-malignant cell lines do not. We speculate that the differences between the high- and low-malignant cells in the expression of cell surface laminin-like antigens contribute to the dissimilarities in attachment and spreading capacity. These differences may also contribute to the dissimilarity between these cells in malignant potential.
从三种不同的甲基胆蒽诱导的小鼠纤维肉瘤中分离出高恶性细胞系和低恶性细胞系，检测它们在无血清条件下以及有血清存在时附着于塑料培养皿和胶原包被培养皿的能力。来自三个高恶性细胞系的大多数细胞在所有条件下都能附着并铺展。到72小时时，细胞数量显著增加，表明至少一些细胞已经进行了分裂（即使在无血清的情况下）。相比之下，来自三个低恶性细胞系的细胞在无血清或存在0.1%血清时，在塑料或胶原底物上附着和铺展的较少。然而，当每培养皿加入15微克层粘连蛋白并与低恶性细胞一起培养时，它们随后在塑料和胶原包被的培养皿上附着并铺展。先前的研究表明，高恶性细胞系表达与层粘连蛋白交叉反应的细胞表面抗原，而低恶性细胞系则不表达。我们推测，高恶性和低恶性细胞在细胞表面层粘连蛋白样抗原表达上的差异导致了附着和铺展能力的不同。这些差异也可能导致这些细胞在恶性潜能方面的差异。
