Seitz-Mayr G, Wolf K
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2618-22. doi: 10.1073/pnas.79.8.2618.
We report the isolation and characterization of a mutator mutant in the fission yeast Schizosaccharomyces pombe. This mutator is of extrachromosomal, very likely mitochondrial, inheritance and acts exclusively on mitochondrial mtDNA. It greatly enhances the frequency of spontaneous mitochondrial drug-resistance mutants compared to the wild type, but it is not obligatory for their occurrence. In contrast, mitochondrial respiratory deficient mutants can only be isolated from mutator strains. It could be shown that this mutator induces point mutations as well as deletions in the mitochondrial genome which lead to respiratory deficiency. This mutator might prove to have a novel function encoded by the mtDNA.
我们报道了粟酒裂殖酵母中一个突变体的分离与鉴定。这个突变体具有染色体外遗传特性，很可能是线粒体遗传，并且仅作用于线粒体DNA（mtDNA）。与野生型相比，它极大地提高了自发线粒体耐药突变体的频率，但这些突变体的出现并非必须依赖于它。相反，线粒体呼吸缺陷突变体只能从携带该突变体的菌株中分离得到。研究表明，这个突变体可在线粒体基因组中诱导点突变和缺失，进而导致呼吸缺陷。这个突变体可能具有由线粒体DNA编码的新功能。
