Wolf K, Lang B, Burger G, Kaudewitz F
Mol Gen Genet. 1976 Feb 27;144(1):75-81.
In contrast to the wild-type, mutant [ANTr8] is able spontaneously to throw off stable respiratory deficient mutants. The frequency of these mutants is considerably enhanced by treatment with ethidium bromide (EB) or the azo-dye Janus green (JG). An unstable cell state with a petite-like phenotype is found in both mutant [ANTr8] and wild-type after EB-treatment. However, only in the mutant is this unstable cell state followed by the appearance of stable respiratory deficient (RD) mutants. Formation of microcolonies is observed both in [ANTr8] and wild-type. RD mutants were isolated after EB treatment. Three of them (mit-12, mit-25, and mit-30) were analyzed and mit-25 characterized in more detail.
与野生型相比，突变体[ANTr8]能够自发地产生稳定的呼吸缺陷型突变体。用溴化乙锭（EB）或偶氮染料詹纳斯绿（JG）处理可显著提高这些突变体的频率。经EB处理后，在突变体[ANTr8]和野生型中均发现了具有小菌落样表型的不稳定细胞状态。然而，只有在突变体中，这种不稳定的细胞状态之后才会出现稳定的呼吸缺陷（RD）突变体。在[ANTr8]和野生型中均观察到微菌落的形成。经EB处理后分离出RD突变体。对其中三个（mit-12、mit-25和mit-30）进行了分析，并对mit-25进行了更详细的表征。
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