Akiyoshi T, Koba F, Kawaguchi M, Miyazaki S, Tsuji H
J Natl Cancer Inst. 1982 Aug;69(2):345-7.
With the use of indirect macrophage migration inhibition (MMI) test, the MMI response to 3-M KCl extract from gastric carcinoma or adjacent normal tissue was studied in patients with gastric carcinoma, in patients with other types of carcinoma, and in normal controls. The migration index (MI) of less than 75, which was 2 SD below the mean MI of normal controls, was considered positive. Twelve (23%) of 52 patients with gastric carcinoma showed positive response to autologous tumor extract, whereas only 2 (6%) of 35 patients responded to autologous normal tissue extract (P less than 0.03). Positive response to allogeneic gastric carcinoma extract occurred in 4 (9%) of 47 patients with gastric carcinoma and in none of 25 patients with other types of carcinoma. None of 39 patients with gastric carcinoma and 19 patients with nongastric carcinoma responded to allogeneic normal tissue extract. These results appeared to show a lower degree of sensitivity but a relatively higher degree of specificity in the indirect MMI test, as compared to those in the direct leukocyte migration inhibition test, for detection of the cell-mediated immune response to tumor-associated antigens in patients with gastric carcinoma.
采用间接巨噬细胞游走抑制（MMI）试验，对胃癌患者、其他类型癌症患者及正常对照者，研究其对胃癌或癌旁正常组织3-M KCl提取物的MMI反应。游走指数（MI）小于75（比正常对照者的平均MI低2个标准差）被视为阳性。52例胃癌患者中有12例（23%）对自体肿瘤提取物呈阳性反应，而35例患者中只有2例（6%）对自体正常组织提取物有反应（P<0.03）。47例胃癌患者中有4例（9%）对异体胃癌提取物呈阳性反应，25例其他类型癌症患者均无反应。39例胃癌患者和19例非胃癌患者对异体正常组织提取物均无反应。与直接白细胞游走抑制试验相比，这些结果似乎表明间接MMI试验在检测胃癌患者对肿瘤相关抗原的细胞介导免疫反应时敏感性较低，但特异性相对较高。
