Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashi-ku, Fukuoka, Japan.
Cytotechnology. 2005 Jan;47(1-3):97-105. doi: 10.1007/s10616-005-3759-2.
In the two-stage cell transformation theory, cancer cells first receive initiation, which is mainly caused by DNA damage, and then promotion, which enhances transformation. Murine Balb/c 3T3 cells are widely used for transformation experiments because they lose contact inhibition ability when transformed. Electrolyzed reduced water (ERW), which is produced near a cathode during electrolysis of water, is an alkaline drinking water that is beneficial to health. ERW contains a high concentration of dissolved hydrogen and scavenge reactive oxygen species (ROS), along with a small amount of platinum (Pt) nanoparticles (Pt nps) derived from Pt-coated titanium electrodes. Pt nps stably disperse in aqueous solution for a long time, and convert hydrogen molecules to active hydrogen (atomic hydrogen) that can scavenge ROS. Therefore, ERW supplemented with synthesized Pt nps is a model strong reduced water. This is the first report that ERW supplemented with synthesized Pt nps strongly prevents transformation of Balb/c 3T3 cells. ERW was prepared by electrolysis of 0.002 M NaOH solution using a batch-type electrolysis device. Balb/c 3T3 cells were treated with 3-methyl cholanthrene (MCA) as an initiation substance, followed by treatment with phorbol-12-myristate-13-acetate (PMA) as a promotion substance. MCA/PMA-induced formation of a transformation focus was strongly suppressed by ERW supplemented with Pt nps but not by ERW or Pt nps individually. ERW supplemented with Pt nps suppressed transformation at the promoter stage, not at initiation, suggesting that ERW supplemented with Pt nps suppressed the PMA-induced augmentation of intracellular ROS. ERW supplemented with Pt nps is a potential new antioxidant against carcinogenesis.
在两阶段细胞转化理论中，癌细胞首先接受引发，主要由 DNA 损伤引起，然后是促进，增强转化。鼠 Balb/c 3T3 细胞广泛用于转化实验，因为它们在转化时失去接触抑制能力。电解还原水（ERW）是在水电解过程中阴极附近产生的一种碱性饮用水，对健康有益。ERW 含有高浓度的溶解氢和活性氧（ROS）清除剂，以及少量源自涂铂钛电极的铂纳米颗粒（Pt nps）。Pt nps 在水溶液中稳定分散很长时间，并将氢分子转化为可以清除 ROS 的活性氢（原子氢）。因此，补充合成 Pt nps 的 ERW 是一种模型强还原水。这是第一个报告补充合成 Pt nps 的 ERW 强烈防止 Balb/c 3T3 细胞转化的报告。ERW 通过使用批处理式电解装置电解 0.002 M NaOH 溶液制备。用 3-甲基胆蒽（MCA）作为引发物质处理 Balb/c 3T3 细胞，然后用佛波醇-12-肉豆蔻酸-13-醋酸酯（PMA）作为促进物质处理。补充有 Pt nps 的 ERW 强烈抑制 MCA/PMA 诱导的转化焦点形成，但 ERW 或 Pt nps 单独处理则没有。补充有 Pt nps 的 ERW 在促进阶段抑制转化，而不在引发阶段，这表明补充有 Pt nps 的 ERW 抑制了 PMA 诱导的细胞内 ROS 增加。补充有 Pt nps 的 ERW 是一种潜在的新型抗氧化剂，可预防致癌作用。
