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纤细无脊椎草履虫耐药突变体的分离与鉴定

Isolation and characterization of drug resistant mutants of Crithidia fasciculata.

作者信息

Hughes D E, Schneider C A, Simpson L

出版信息

J Parasitol. 1982 Aug;68(4):642-9.

PMID:6956705
Abstract

Mutants of Crithidia fasciculata, resistant to Actinomycin D, Tubercidin, Crystal Violet, 6-Azauridine, and 5-Fluorouracil were obtained and characterized. The mutants were stable when maintained under nonselective conditions, and could be grouped in several subclasses on the basis of cross-resistance to other drugs. Actinomycin D- and Crystal Violet-resistant mutants appeared spontaneously whereas the others appeared only after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Analysis with a cell sorter indicated no large scale meiotic changes in ploidy under the various culture conditions employed. These mutants may provide suitable, selectable, genetic markers for use in studying the possibility of a sexual mating process in Crithidia.

摘要

获得并鉴定了对放线菌素D、杀结核菌素、结晶紫、6-氮尿苷和5-氟尿嘧啶具有抗性的纤细无脊锥虫突变体。这些突变体在非选择性条件下保持稳定，并且可以根据对其他药物的交叉抗性分为几个亚类。对放线菌素D和结晶紫具有抗性的突变体自发出现，而其他突变体仅在用N-甲基-N'-硝基-N-亚硝基胍诱变后出现。使用细胞分选仪分析表明，在所采用的各种培养条件下，倍性没有大规模的减数分裂变化。这些突变体可能为研究纤细无脊锥虫有性交配过程的可能性提供合适的、可选择的遗传标记。

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引用本文的文献

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The 28S-18S rDNA intergenic spacer from Crithidia fasciculata: repeated sequences, length heterogeneity, putative processing sites and potential interactions between U3 small nucleolar RNA and the ribosomal RNA precursor.来自纤细短膜虫的28S-18S rDNA基因间隔区：重复序列、长度异质性、假定的加工位点以及U3小核仁RNA与核糖体RNA前体之间的潜在相互作用
Nucleic Acids Res. 2000 Sep 15;28(18):3452-61. doi: 10.1093/nar/28.18.3452.
2
Occurrence of N-acetyl- and N-O-diacetyl-neuraminic acid derivatives in wild and mutant Crithidia fasciculata.野生型和突变型 fasciculata 锥虫中 N-乙酰神经氨酸和 N-O-二乙酰神经氨酸衍生物的存在情况
Parasitol Res. 1995;81(5):426-33. doi: 10.1007/BF00931505.
3
Interaction of aminoglycosides and ionophores in the killing of Crithidia fasciculata.
Z Parasitenkd. 1985;71(6):699-704. doi: 10.1007/BF00926795.
4
High-efficiency clonal growth of bloodstream- and insect-form Trypanosoma brucei on agarose plates.布氏锥虫血液型和昆虫型在琼脂糖平板上的高效克隆生长
Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8818-21. doi: 10.1073/pnas.89.18.8818.
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Surface charge and hydrophobicity of wild and mutant Crithidia fasciculata.野生型和突变型纤细短膜虫的表面电荷与疏水性
Cell Biophys. 1992 Feb;20(1):69-79. doi: 10.1007/BF02782655.