Samovar A G, Krylov M V
Parazitologiia. 1987 Nov-Dec;21(6):701-9.
Strains resistant to 100 mkg/ml of cycloheximide (ChxR100) and to 2.5 mg/ml of chloramphenicol (CapR2.5) were used in studies for the presence of genetic interchange in C. oncopelti. Phenotypes of these strains were stable on the nonselective medium and specific. Strains ChxR100 and CapR2.5 were mixed in equal proportions, cultivated during 7 days and tested. Under consecutive effect of inhibitors the culture of strains mixture and clones isolated from it exhibited double resistance. Phenotypes of recombinant clones were stable on the nonselective medium during, at the minimum, 70 to 90 cell generations. At the same conditions control cultures of parental strains and clones did not exhibit resistance that excludes the possibility of origin of cells resistant to both antibiotics from Crithidia of one of the parental strains as a result of secondary mutation. Thus, the results obtained show convincingly the presence of genetic interchange between flagellates of ChxR100 and CapR2.5 strains.
对100微克/毫升放线菌酮（ChxR100）和2.5毫克/毫升氯霉素（CapR2.5）具有抗性的菌株被用于研究棉红蝽锥虫（C. oncopelti）中基因交换的存在情况。这些菌株的表型在非选择性培养基上是稳定且特异的。将ChxR100和CapR2.5菌株按等比例混合，培养7天并进行检测。在抑制剂的连续作用下，菌株混合物培养物及其分离出的克隆表现出双重抗性。重组克隆的表型在非选择性培养基上至少在70至90个细胞世代中保持稳定。在相同条件下，亲本菌株和克隆的对照培养物未表现出抗性，这排除了由于二次突变而从其中一个亲本菌株的克氏锥虫（Crithidia）产生对两种抗生素均具有抗性的细胞的可能性。因此，所获得的结果令人信服地表明ChxR100和CapR2.5菌株的鞭毛虫之间存在基因交换。
