Cochet C, Créau-Goldberg N, Turleau C, De Grouchy J
Cytogenet Cell Genet. 1982;33(3):213-21. doi: 10.1159/000131757.
The karyotype of microcebus murinus (MIM) (lemuridae) is considered by Dutrillaux (1979) as the closest to the karyotype ancestral to all primates. A large number of homoeologies exists between the banding patterns of MIM chromosomes and those of man (HSA). We report a comparison of the gene maps of these two species which confirms most of these homoeologies. Fifteen cell hybrids were obtained by fusing MIM fibroblasts and an HPRT- Chinese hamster cell line. Twenty-seven enzyme markers were investigated. The following assignments were demonstrated: NP to chromosome MIM 2, homoeologous to HSA 14; the syntenic group PGD-ENO1-PGM1 to MIM 3, homoeologous to HSA 1p; LDHA to MIM 5, homoeologous to HSA 11; Me1 to MIM 6, homoeologous to HSA 6; the syntenic group LDHB-CS-PEPB-ENO2-TPI to MIM 7, homoeologous to HSA 12; the syntenic group AK1-AK3 to MIM 10, which we considered to be homoeologous to HSA 9 (we do not consider MIM 9 to be homoeologous to HSA 9, as does Dutrillaux, 1979); GOT1 to MIM 15, homoeologous to HSA 10; the syntenic group HPRT-G6PD-PGK-GLA to MIM X. Synteny dissociation in three hybrids suggests closer linkage between G6PD and HPRT than between PGK-GLA and HPRT. Three syntenic groups, known in man, were confirmed in MIM but could not be assigned with full confidence: ACP1-MDH1, MP1-PKM2, and PEPD-GPI. GUK1 and PEPC, known to be syntenic in man, were found to be asyntenic in MIM and could not be assigned. PGM2 and SOD1 could not be assigned. A comparison of these gene assignments with those known in Cebus capucinus showed a remarkable homoeology for six chromosomes of the two species.
杜特里洛（1979年）认为，小鼠狐猴（MIM）（狐猴科）的核型与所有灵长类动物的祖先核型最为接近。小鼠狐猴染色体的带型与人（HSA）的带型之间存在大量同源性。我们报告了对这两个物种基因图谱的比较，证实了其中大部分同源性。通过将小鼠狐猴成纤维细胞与一种次黄嘌呤磷酸核糖转移酶缺陷型中国仓鼠细胞系融合，获得了15个细胞杂种。研究了27种酶标记。得到了以下定位结果：NP定位于小鼠狐猴第2号染色体，与人类第14号染色体同源；同线群PGD - ENO1 - PGM1定位于小鼠狐猴第3号染色体，与人类1p同源；LDHA定位于小鼠狐猴第5号染色体，与人类第11号染色体同源；Me1定位于小鼠狐猴第6号染色体，与人类第6号染色体同源；同线群LDHB - CS - PEPB - ENO2 - TPI定位于小鼠狐猴第7号染色体，与人类第12号染色体同源；同线群AK1 - AK3定位于小鼠狐猴第10号染色体，我们认为它与人类第9号染色体同源（我们不像杜特里洛1979年那样认为小鼠狐猴第9号染色体与人类第9号染色体同源）；GOT1定位于小鼠狐猴第15号染色体，与人类第10号染色体同源；同线群HPRT - G6PD - PGK - GLA定位于小鼠狐猴X染色体。三个杂种中的同线解离表明，G6PD与HPRT之间的连锁比PGK - GLA与HPRT之间的连锁更紧密。在小鼠狐猴中证实了人类已知的三个同线群，但无法完全确定其定位：ACP1 - MDHl、MP1 - PKM2和PEPD - GPI。已知在人类中同线的GUK1和PEPC，在小鼠狐猴中被发现不同线，无法进行定位。PGM2和SOD1也无法进行定位。将这些基因定位与卷尾猴已知的定位进行比较，发现这两个物种的六条染色体具有显著的同源性。
