Vaughan A T, Bateman W J, Brown G, Cowan J
Int J Nucl Med Biol. 1982;9(3):167-71. doi: 10.1016/0047-0740(82)90091-2.
A monoclonal antibody with a broad anti-human leucocyte specificity, designated BK 19.45 and the plant lectin Concanavalin A have been labelled with the alpha-emitting cyclotron produced radiohalogen astatine-211. Both human and murine tumour cell lines and human leukemic bone marrow samples have been specifically labelled with these radioactive proteins. In all cases the amount of 211At bound to the cells is directly correlated with a decrease in cellular reproductive potential as shown by the ability of the cells to proliferate in a clonogenic assay. For the labelled monoclonal antibody experiments, the radiation dose needed to yield 37% cell survival, the D37 dose, may be achieved with an average of 12 211At atoms/cell.
一种具有广泛抗人白细胞特异性的单克隆抗体，命名为BK 19.45，以及植物凝集素伴刀豆球蛋白A，已用α发射回旋加速器产生的放射性卤素砹-211进行了标记。人和鼠的肿瘤细胞系以及人白血病骨髓样本都已被这些放射性蛋白质特异性标记。在所有情况下，与细胞结合的砹-211的量与细胞繁殖潜能的降低直接相关，这通过细胞在克隆形成试验中的增殖能力得以体现。对于标记单克隆抗体的实验，产生37%细胞存活率所需的辐射剂量，即D37剂量，平均每个细胞有12个砹-211原子时即可达到。
