Zwolska-Kwiek Z, Kwiek S
Z Erkr Atmungsorgane. 1978 Mar;150(3):279-86.
A technique for the autoradiography of colonies of mycobacteria on membrane filters has been evolved. The method enables studies on the effect of various factors on the incorporation of radioisotopes by mycobacteria. In this study the effect of INH, SM and RMP on the incorporation of 32P by mycobacteria cultivated in the form of microcolonies on membrane filters was examined. 30 minute incubation of Myc. phlei or 10 hour incubation of Myc. H37Ra with these drugs caused visible inhibition of 32P incorporation by drug-sensitive mycobacteria.
一种用于对膜滤器上的分枝杆菌菌落进行放射自显影的技术已经研发出来。该方法能够研究各种因素对分枝杆菌摄取放射性同位素的影响。在本研究中，检测了异烟肼（INH）、链霉素（SM）和利福平（RMP）对在膜滤器上以微菌落形式培养的分枝杆菌摄取32P的影响。将草分枝杆菌孵育30分钟或将结核杆菌H37Ra与这些药物孵育10小时，会导致药物敏感的分枝杆菌对32P的摄取受到明显抑制。
