Siddique M A, Rahman K M, Muazzam N, Hossain T, Islam K M, Rahman M A, Chowdhury F A, Ali C M
Deptt. of Microbiology, Dhaka Medical College, Bangladesh.
Bangladesh Med Res Counc Bull. 1995 Apr;21(1):18-23.
The study was carried out to investigate the primary drug resistance pattern of tubercle bacilli isolated from the pulmonary tuberculosis patient attended in Shyamoli TB clinic, Dhaka. Sputum from 961 suspected tuberculous patients were randomly collected and stained by Ziehl-Neelsen (Z.N) stain. 135 were microscopically positive for Acid Fast Bacilli (AFB). Among them 30 patients were excluded from the study as they received antitubercular treatment before. So only 105 microscopically positive cases were cultured on Lowenstein-Jensen (L-J) media and 100 showed pure growth and rest 5 were contaminated with fungus. These 100 cases were studied on 4 antitubercular drugs. Out of these 100 isolates, 91 were M. tuberculosis and rest 9 in the nonchromogen group of mycobacteria other than tuberculosis (MOTT) species. Among 91 M. tuberculosis species, 89 (97.80%) to Isoniazid (INH), 73 (80.21%) to Rifampicin (RMP) and 91 (100%) to Streptomycin (SM) and Ethambutol (ETHM) were sensitive. Of the 9 MOTT species, 4 (44.44%) to SM, 7 (77.78%) to ETHM were sensitive and all (100%) were resistant to INH and RMP. Among the 100 isolates, 27 (18 M. tuberculosis and 9 MOTT) were resistant to 4 drugs either single or in combination. Of the 18 (66.67%) M. tuberculosis species, 16 (59.26%) to RMP, and 2 (7.41%) to RMP and INH were resistant. Of the 9 (33.33%) MOTT species, 4 (14.81%) to RMP and INH, 3 (11.11%) to RMP, INH and SM and 2 (7.41%) to RMP, INH, SM and ETHM were resistant.
开展该研究旨在调查从达卡Shyamoli结核病诊所就诊的肺结核患者中分离出的结核杆菌的原发性耐药模式。随机收集了961名疑似结核病患者的痰液，并用萋-尼（Z.N）染色法进行染色。135例痰涂片抗酸杆菌（AFB）镜检阳性。其中30例患者因之前接受过抗结核治疗而被排除在研究之外。因此，仅对105例镜检阳性病例在罗-琴（L-J）培养基上进行培养，100例显示纯培养生长，其余5例被真菌污染。对这100例病例使用4种抗结核药物进行研究。在这100株分离株中，91株为结核分枝杆菌，其余9株属于非结核分枝杆菌（MOTT）的非产色菌群。在91株结核分枝杆菌中，89株（97.80%）对异烟肼（INH）敏感，73株（80.21%）对利福平（RMP）敏感，91株（100%）对链霉素（SM）和乙胺丁醇（ETHM）敏感。在9株MOTT中，4株（44.44%）对SM敏感，7株（77.78%）对ETHM敏感，所有菌株（100%）对INH和RMP耐药。在100株分离株中，27株（18株结核分枝杆菌和9株MOTT）对4种药物单药或联合耐药。在18株（66.67%）结核分枝杆菌中，16株（59.26%）对RMP耐药，2株（7.41%）对RMP和INH耐药。在9株（33.33%）MOTT中，4株（14.81%）对RMP和INH耐药，3株（11.11%）对RMP、INH和SM耐药，2株（7.41%）对RMP、INH、SM和ETHM耐药。
