Cameron D J, Churchill W H
Jpn J Exp Med. 1982 Feb;52(1):9-16.
Five paired human cell lines consisting of either skin fibroblasts or tumor obtained from patients with osteogenic sarcoma were studied for their susceptibility to macrophage mediated cytotoxicity. In two of the matched pairs, the malignant cell lines were susceptible to the effects of cytotoxic macrophages, whereas normal targets were not. In two other pairs, both malignant and normal targets were resistant to the effects of cytotoxic macrophages. In the fifth pair, both targets were sensitive to the effects of the macrophages. Cell surface sialic acid content of the five matched pairs and several previously studied targets was determined. It was found to correlate with susceptibility to macrophage mediated cytotoxicity. The resistant normal cell lines contained approximately 50% less sialic acid than did the sensitive tumor cell lines. Furthermore, the three resistant tumor lines also had cell surface sialic acid content in the range of the normal resistant targets. Sensitive tumor cells with high cell surface sialic acid, after neuraminidase treatment, became resistant to the effects of cytotoxic macrophages and the effect of the neuraminidase treatment was blocked by addition of sialic acid to the incubation mixture. Thus, it appears that susceptibility to macrophage mediated cytotoxicity is associated with increased levels of sialic acid on the cell surface membranes.
对五对来自骨肉瘤患者的人类细胞系（由皮肤成纤维细胞或肿瘤组成）进行了研究，以观察它们对巨噬细胞介导的细胞毒性的敏感性。在两对匹配的细胞系中，恶性细胞系对细胞毒性巨噬细胞的作用敏感，而正常靶细胞则不敏感。在另外两对中，恶性和正常靶细胞均对细胞毒性巨噬细胞的作用具有抗性。在第五对中，两个靶细胞均对巨噬细胞的作用敏感。测定了五对匹配细胞系以及几个先前研究过的靶细胞的细胞表面唾液酸含量。发现其与对巨噬细胞介导的细胞毒性的敏感性相关。抗性正常细胞系所含的唾液酸比敏感肿瘤细胞系少约50%。此外，三个抗性肿瘤系的细胞表面唾液酸含量也在正常抗性靶细胞的范围内。细胞表面唾液酸含量高的敏感肿瘤细胞经神经氨酸酶处理后，对细胞毒性巨噬细胞的作用产生抗性，并且在孵育混合物中添加唾液酸可阻断神经氨酸酶处理的作用。因此，似乎对巨噬细胞介导的细胞毒性的敏感性与细胞膜表面唾液酸水平的升高有关。
