局部测量的细胞变形性对细胞位置、温度和细胞松弛素B的依赖性。
Dependence of locally measured cellular deformability on position on the cell, temperature, and cytochalasin B.
作者信息
Petersen N O, McConnaughey W B, Elson E L
出版信息
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5327-31. doi: 10.1073/pnas.79.17.5327.
Abstract
We describe an approach to exploring cell surface-cytoskeleton interactions through direct measurements of the mechanical resistance of living cells to locally applied forces. These measurements are sensitive to variations in structure across the cell and at various depths below its surface. We find that local cellular deformability depends on the temperature and on the integrity of the cytoskeleton. Cytochalasin B increases the deformability of all regions of the cell except the nucleus.
摘要
我们描述了一种通过直接测量活细胞对局部施加力的机械阻力来探索细胞表面与细胞骨架相互作用的方法。这些测量对细胞整体结构及其表面以下不同深度处结构的变化很敏感。我们发现局部细胞变形能力取决于温度和细胞骨架的完整性。细胞松弛素B会增加细胞除细胞核外所有区域的变形能力。
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