Viinikka L, Ylikorkala O
Prostaglandins Leukot Med. 1982 Jul;9(1):1-7. doi: 10.1016/0262-1746(82)90067-1.
While developing a radioimmunoassay for the measurement of 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) in human plasma, we obtained two antibodies which had apparently similar cross-reactivities with 22 other prostaglandins and related compounds. However, in radioimmunoassay, use of one of the antibodies gave results which were about double the results obtained with the other. The results obtained by both antibodies correlated significantly with each other according to the equation y = 0.43x - 13.0 pg/ml, R = 0.89, n = 58 (p less than 0.001). The increments of plasma 6-keto-PGF1 alpha after an intravenous infusion of prostacyclin (PGI2) also correlated with the dose of PGI2 (R = 0.93 and 0.96, n = 24, p less than 0.001) in both cases, and the increments obtained by the use of both antibodies correlated with each other (R = 0.94, n = 24, p less than 0.001). The increments were, however, of different magnitude. These differences can be best explained by assuming different immunoreactivity of the two antibodies with metabolites of PGI2 other than 6-keto-PGF1 alpha, or with the two known stereochemical configuration of 6-keto-PGF1 alpha.
在开发一种用于测量人血浆中6-酮-前列腺素F1α（6-keto-PGF1α）的放射免疫测定法时，我们获得了两种抗体，它们与其他22种前列腺素及相关化合物的交叉反应性明显相似。然而，在放射免疫测定中，使用其中一种抗体得到的结果约为使用另一种抗体得到结果的两倍。根据y = 0.43x - 13.0 pg/ml的方程，两种抗体得到的结果彼此显著相关，R = 0.89，n = 58（p < 0.001）。在静脉输注前列环素（PGI2）后，血浆6-keto-PGF1α的增量在两种情况下也都与PGI2的剂量相关（R = 0.93和0.96，n = 24，p < 0.001），并且使用两种抗体得到的增量彼此相关（R = 0.94，n = 24，p < 0.001）。然而，增量的大小不同。通过假设这两种抗体与6-keto-PGF1α以外的PGI2代谢物或与6-keto-PGF1α的两种已知立体化学构型具有不同的免疫反应性，可以最好地解释这些差异。
