使用[125I]-6-酮前列环素F1α-酪胺对6-酮前列环素F1α进行放射免疫测定

[A radioimmunoassay of 6-keto PG F1 alpha using [125I]-6-keto PG F1 alpha-tyramide].

作者信息

Sekine M

出版信息

Nihon Naibunpi Gakkai Zasshi. 1985 Jan 20;61(1):1-13. doi: 10.1507/endocrine1927.61.1_1.

DOI:10.1507/endocrine1927.61.1_1

Abstract

We established a radioimmunoassay of 6-keto PG F1 alpha, using 125I-6-keto PG F1 alpha-tyramide, which enabled us more easily to monitor the plasma levels of prostacyclin in clinical fields. Antibodies against 6-keto PG F1 alpha were obtained from rabbits immunized with 6-keto PG F1 alpha-BSA complex, which could be used for RIA at a final dilution of 1:648,000. The detection limit of 6-keto PG F1 alpha was 50-1000 pg/ml. The cross reactivities between PG I2 and PG F1 alpha were 26.6% and 1.62% respectively, but those between other prostaglandins were less than 1%. Using this radioimmunoassay with 125I-6-keto PG F1 alpha-tyramide, plasma levels of 6-keto PG F1 alpha were measured. The recovery rate by this assay was 92.9%, and the intra- and inter-assay coefficients of variation determined were 9.9% and 27.1%, respectively. The mean of plasma levels of 6-keto PG F1 alpha revealed 126.2 +/- 75.3 (mean +/- S.D.) pg/ml in 46 healthy Japanese males, 122.7 +/- 85.2 pg/ml in 60 females, and 124.2 +/- 81.3 pg/ml in 106 Japanese of both sexes.

摘要

我们使用125I-6-酮-前列腺素F1α-酪胺建立了一种6-酮-前列腺素F1α的放射免疫测定法，这使我们能够更轻松地在临床领域监测前列环素的血浆水平。抗6-酮-前列腺素F1α的抗体是从用6-酮-前列腺素F1α-牛血清白蛋白复合物免疫的兔子中获得的，该抗体在最终稀释度为1:648,000时可用于放射免疫测定。6-酮-前列腺素F1α的检测限为50-1000 pg/ml。前列环素I2与前列腺素F1α之间的交叉反应率分别为26.6%和1.62%，但其他前列腺素之间的交叉反应率小于1%。使用这种125I-6-酮-前列腺素F1α-酪胺放射免疫测定法测量了6-酮-前列腺素F1α的血浆水平。该测定法的回收率为92.9%，测定内和测定间的变异系数分别为9.9%和27.1%。46名健康日本男性的6-酮-前列腺素F1α血浆水平平均值为126.2±75.3（平均值±标准差）pg/ml，60名女性为122.7±85.2 pg/ml，106名日本男女混合样本为124.2±81.3 pg/ml。