Johnson G A, Barsuhn K J, McCall J M
Biochem Pharmacol. 1982 Sep 15;31(18):2949-54. doi: 10.1016/0006-2952(82)90268-4.
The 100,000 g supernatant fraction of rat liver homogenate contains a sulfotransferase activity which catalyzes the sulfation of minoxidil. Synthetic minoxidil N-O sulfate and the enzyme synthesized product had identical chromatographic characteristics on high pressure liquid chromatography. Minoxidil sulfate, which yields minoxidil when treated with sulfatase, was slowly hydrolyzed in water. Several N-oxides of other heterocycles, including several other pyrimidines, triazines and imidazoles, were also substrates for this sulfotransferase.
大鼠肝脏匀浆的100,000g上清液组分含有一种硫酸转移酶活性，可催化米诺地尔的硫酸化反应。合成的米诺地尔N-O硫酸盐与该酶的合成产物在高压液相色谱上具有相同的色谱特征。用硫酸酯酶处理时可产生米诺地尔的硫酸米诺地尔在水中会缓慢水解。包括其他几种嘧啶、三嗪和咪唑在内的其他杂环化合物的几种N-氧化物也是这种硫酸转移酶的底物。
