Searls D B, Edidin M
J Natl Cancer Inst. 1982 Dec;69(6):1311-5.
The murine teratocarcinoma-derived cell line TerC, previously thought to lack products of the major histocompatibility locus H-2, was shown to express low levels of K- and D-end H-2 specificities with the use of a sensitive cytotoxic assay. The assay, based on the ability of antibody and complement to inhibit uptake of the thymidine analogue [125I]5-iodo-2'-deoxyuridine, detected appropriate public and private specificities, as demonstrated with the use of oligospecific antisera and by absorption analyses. A series of clone of TerC varied only slightly in H-2 expression, and there was no tendency for expression to increase with time in culture; thus the low levels of H-2 were not the result of a differentiating subpopulation.
鼠畸胎瘤衍生细胞系TerC，以前被认为缺乏主要组织相容性位点H-2的产物，通过一种灵敏的细胞毒性测定法显示其表达低水平的K和D端H-2特异性。该测定法基于抗体和补体抑制胸苷类似物[125I]5-碘-2'-脱氧尿苷摄取的能力，检测到了合适的公共和私有特异性，这通过使用寡特异性抗血清和吸收分析得以证明。TerC的一系列克隆在H-2表达上仅有轻微差异，且在培养过程中表达没有随时间增加的趋势；因此，低水平的H-2并非分化亚群的结果。
