Lea T, Michaelsen T E, Rasmussen A M
Clin Exp Immunol. 1982 Oct;50(1):139-47.
In vitro transformation of human peripheral blood lymphocytes was induced by a purified group A streptococcal extract rich in streptolysin O (SLO). The stimulation was shown to be largely a general mitogenic effect rather than antigen-selective as a large panel of normal blood donors responded strongly. Further support for a non-specific stimulation was obtained by experiments with cord blood lymphocytes. An antigen-specific response could also be detected as culture supernatants from the stimulation experiments contained immunoglobulins with SLO neutralizing properties. In addition, the total response could partly be inhibited by rabbit heteroantisera against HLA-DR-like antigens supporting the above contention. The stimulatory properties were found to reside in the SLO molecule although results may indicate that the determinants provoking the lymphocyte transformation differ from the determinants possessing the haemolytic properties. Peak response was found on days 4-5. Cell fractionation experiments indicated that only T lymphocytes were stimulated although co-operating B cells and/or monocytes were necessary.
富含链球菌溶血素O（SLO）的纯化A组链球菌提取物可诱导人外周血淋巴细胞的体外转化。结果表明，这种刺激在很大程度上是一种普遍的促有丝分裂效应，而非抗原选择性的，因为大量正常献血者的血液对此反应强烈。通过对脐血淋巴细胞进行实验，进一步证实了这种非特异性刺激。刺激实验的培养上清液中含有具有SLO中和特性的免疫球蛋白，这也能检测到抗原特异性反应。此外，兔抗HLA - DR样抗原异种抗血清可部分抑制总反应，支持了上述观点。虽然结果可能表明引发淋巴细胞转化的决定簇与具有溶血特性的决定簇不同，但发现刺激特性存在于SLO分子中。在第4 - 5天观察到反应峰值。细胞分级分离实验表明，虽然协同作用的B细胞和/或单核细胞是必需的，但只有T淋巴细胞受到刺激。
