Smith G S, Warhurst G, Turnberg L A
Biochim Biophys Acta. 1982 Dec 13;713(3):684-7. doi: 10.1016/0005-2760(82)90331-9.
Methods are described for the parallel assay of prostaglandin E2 synthesis and degradation on a single homogenate. Using these methods, we show that prostaglandin synthesis is located predominantly in the subepithelium whereas the capacity to degrade prostaglandins resides mainly in the epithelial cells. Separation of the epithelial cells into a 'villus to crypt gradient' showed that the capacity to degrade prostaglandins decreased from villus to crypt whereas the responsiveness of the adenylate cyclase to exogenous prostaglandin E2 increased. These findings are discussed in relation to cyclic AMP-mediated, prostaglandin-induced secretion.
本文描述了在单一匀浆上对前列腺素E2合成与降解进行平行测定的方法。使用这些方法，我们发现前列腺素合成主要位于上皮下，而前列腺素降解能力主要存在于上皮细胞中。将上皮细胞分离成“绒毛到隐窝梯度”显示，前列腺素降解能力从绒毛到隐窝逐渐降低，而腺苷酸环化酶对外源性前列腺素E2的反应性增加。结合环磷酸腺苷介导的、前列腺素诱导的分泌对这些发现进行了讨论。
