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[不同冷冻保护剂低温冷冻过程中白血病细胞的免疫原性]

[Immunogenicity of leukemic cells during low-temperature freezing with different cryoprotectors].

作者信息

Bulycheva T I, Smolina N V, Kalinina I A, Ol'shanskaia N V

出版信息

Biull Eksp Biol Med. 1982 Dec;94(12):75-7.

PMID:6960935
Abstract

Immunogenicity of leukemic cells was studied and compared during conservation with the three cryoprotectors, DMSO, DMAC, and PEO. Immunogenicity of leukemic cells was assessed from immune response of the experimental animals to immunization with these cells (the titer of cytotoxic antibodies and lymphocyte cytotoxicity). The data obtained indicate that immunogenicity of leukemic cells conserved with DMAC and PEO does not virtually differ from that of native cells or of cells conserved with DMSO.

摘要

研究并比较了白血病细胞在使用三种冷冻保护剂二甲基亚砜(DMSO)、二甲基乙酰胺(DMAC)和聚环氧乙烷(PEO)保存期间的免疫原性。通过实验动物对这些细胞免疫接种的免疫反应(细胞毒性抗体滴度和淋巴细胞细胞毒性)来评估白血病细胞的免疫原性。所获得的数据表明,用DMAC和PEO保存的白血病细胞的免疫原性与天然细胞或用DMSO保存的细胞的免疫原性几乎没有差异。

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