Stern J H, Lisman J E
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7580-4. doi: 10.1073/pnas.79.23.7580.
The internal dialysis technique has been applied to Limulus ventral photoreceptors. This method potentially allows quantitative control of the concentration of diffusible molecules within living cells. During dialysis, Limulus photoreceptors retained their ability to respond to light. Under conditions of dim illumination, responses were normal for close to an hour. In bright light, abnormalities developed more rapidly. The reversible effects of raising the dialysate Mg2+ concentration and the entrance of rhodamine-labeled albumin into cells shows that the dialysis method is useful for assaying the effects of small or large molecules on visual transduction. This method has been used to examine the effects of nucleotide triphosphates and cyclic nucleotides. The results show that nucleotide triphosphates (5-10 mM) are required to maintain a low rate of spontaneous quantum bumps. The importance of cyclic nucleotides in transduction is less clear; the light response was reduced by either cGMP or cAMP but only at very high concentrations (10 mM).
内透析技术已应用于鲎腹侧光感受器。该方法有可能对活细胞内可扩散分子的浓度进行定量控制。在透析过程中，鲎光感受器保留了对光作出反应的能力。在弱光条件下，近一小时内反应正常。在强光下，异常情况出现得更快。提高透析液中Mg2+浓度以及罗丹明标记的白蛋白进入细胞的可逆效应表明，透析方法可用于测定小分子或大分子对视觉转导的影响。该方法已用于研究三磷酸核苷酸和环核苷酸的作用。结果表明，需要三磷酸核苷酸（5 - 10 mM）来维持低水平的自发量子突增率。环核苷酸在转导中的重要性尚不清楚；cGMP或cAMP均可降低光反应，但仅在非常高的浓度（10 mM）下才会出现这种情况。
