从鲎腹侧光感受器的分离膜片中获得的光依赖通道可被环鸟苷酸（cGMP）打开。
Light-dependent channels from excised patches of Limulus ventral photoreceptors are opened by cGMP.
作者信息
Bacigalupo J, Johnson E C, Vergara C, Lisman J E
机构信息
Department of Biology, Faculty of Sciences, University of Chile, Santiago.
出版信息
Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7938-42. doi: 10.1073/pnas.88.18.7938.
Abstract
The identity of the second messenger that directly activates the light-dependent conductance in invertebrate photoreceptors remains unclear; the available evidence provides some support for cGMP and Ca2+. To resolve this issue we have applied these second messengers to membrane patches excised from the light-sensitive lobe of Limulus ventral photoreceptors. Our results show that these patches contain channels that can be opened by cGMP, but not by Ca2+. These cGMP-activated channels closely resemble the channels activated by light in cell-attached patches. This evidence suggests that cGMP is the messenger that opens the light-dependent channel in invertebrate photoreceptors.
摘要
直接激活无脊椎动物光感受器中光依赖性电导的第二信使的身份仍不清楚；现有证据为环鸟苷酸（cGMP）和钙离子（Ca2+）提供了一些支持。为了解决这个问题，我们将这些第二信使应用于从鲎腹侧光感受器的光敏叶上切除的膜片。我们的结果表明，这些膜片中含有可被cGMP打开但不能被Ca2+打开的通道。这些cGMP激活的通道与细胞贴附膜片中被光激活的通道非常相似。这一证据表明，cGMP是打开无脊椎动物光感受器中光依赖性通道的信使。
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