Sasaki T, Nakagawa K, Higashi S
Arch Oral Biol. 1982;27(10):897-904. doi: 10.1016/0003-9969(82)90048-6.
Fifteen kittens were perfused with 2.5 per cent glutaraldehyde or modified Karnovsky fixative. Distribution and structural features of the three kinds of intercellular junctions; gap junctions, macular tight junctions and desmosome-like junctions were clarified by correlated observations using thin sections and freeze-fracture replicas. Distal junctional complexes of the odontoblasts were composed of both gap and macular tight junctions and sealed extracellular spaces incompletely, because of the poor sealing capacities of macular tight junctions. Therefore, there was no predentine-pulp barrier in the odontoblast layer. Except for the junctional complex, no tight junction was observed in the odontoblasts. Gap junctions and desmosome-like junctions were found between adjacent odontoblasts and between odontoblasts and neighbouring pulp cells. Gap junctions were similar to those of many other tissues but the desmosome-like junctions were different from mature desmosomes in the epithelial cells and showed immature features. Each intercellular junction of odontoblasts is considered to form a site of intercellular communication and cell-to-cell attachment.
15只小猫用2.5%的戊二醛或改良的卡诺夫斯基固定液进行灌注。通过薄切片和冷冻断裂复制品的相关观察，阐明了三种细胞间连接（缝隙连接、斑状紧密连接和桥粒样连接）的分布和结构特征。成牙本质细胞的远侧连接复合体由缝隙连接和斑状紧密连接组成，由于斑状紧密连接的封闭能力较差，细胞外间隙未被完全封闭。因此，在成牙本质细胞层不存在前期牙本质-牙髓屏障。除连接复合体外，在成牙本质细胞中未观察到紧密连接。在相邻的成牙本质细胞之间以及成牙本质细胞与相邻的牙髓细胞之间发现了缝隙连接和桥粒样连接。缝隙连接与许多其他组织的相似，但桥粒样连接与上皮细胞中的成熟桥粒不同，表现出不成熟的特征。成牙本质细胞的每个细胞间连接都被认为形成了细胞间通讯和细胞间附着的位点。
