铍中毒。酪蛋白激酶1的选择性抑制。
Beryllium toxicity. The selective inhibition of casein kinase 1.
作者信息
Cummings B, Kaser M R, Wiggins G, Ord M G, Stocken L A
出版信息
Biochem J. 1982 Oct 15;208(1):141-6. doi: 10.1042/bj2080141.
Abstract
- Cyclic AMP-independent casein kinase 1 in liver cytoplasm and nuclei was inhibited by Be2+ in vitro (Ki 2.5 microM and 29 microM respectively). Casein kinase 2 (phosvitin kinase) and cyclic AMP-dependent protein kinase were unaffected. 2. The inhibition of casein kinase 1 by Be2+ was competitive with respect to the protein substrate; at non-saturating concentrations of casein, inhibition was non-competitive with respect to ATP. 3. In rats given LD50 doses of Be2+ 24 h before death, the activities of cytoplasmic and nuclear casein kinase 1 in livers from partially hepatectomized animals were diminished approx. 50%; with intact rats, nuclear casein kinase 1 was inhibited at concentrations of casein less than the Km.
摘要
- 体外实验中，Be2+可抑制肝细胞质和细胞核中不依赖环磷酸腺苷（cAMP）的酪蛋白激酶1（Ki分别为2.5微摩尔/升和29微摩尔/升）。酪蛋白激酶2（磷蛋白激酶）和依赖环磷酸腺苷的蛋白激酶未受影响。2. Be2+对酪蛋白激酶1的抑制作用在蛋白质底物方面具有竞争性；在酪蛋白非饱和浓度下，对三磷酸腺苷（ATP）而言抑制作用无竞争性。3. 在死亡前24小时给予半数致死剂量Be2+的大鼠中，部分肝切除动物肝脏中细胞质和细胞核酪蛋白激酶1的活性降低约50%；对于完整大鼠，当酪蛋白浓度低于米氏常数（Km）时，细胞核酪蛋白激酶1受到抑制。
相似文献
1
Beryllium toxicity. The selective inhibition of casein kinase 1.铍中毒。酪蛋白激酶1的选择性抑制。
Biochem J. 1982 Oct 15;208(1):141-6. doi: 10.1042/bj2080141.
2
Inhibition of nuclear-protein phosphorylation in vitro by beryllium.铍在体外对核蛋白磷酸化的抑制作用。
Biosci Rep. 1983 Oct;3(10):955-62. doi: 10.1007/BF01140665.
3
Cyclic AMP-dependent histone-specific nucleoplasmic protein kinase from rat liver.来自大鼠肝脏的环磷酸腺苷依赖性组蛋白特异性核质蛋白激酶
Biochem J. 1978 Apr 1;171(1):123-35. doi: 10.1042/bj1710123.
4
Protein kinase activities in rat liver nuclei: effects of age and partial hepatectomy.大鼠肝细胞核中的蛋白激酶活性：年龄和部分肝切除的影响。
Biosci Rep. 1986 Jun;6(6):565-71. doi: 10.1007/BF01114953.
5
[Effect of heparin, spermidine and Be2+ ions on the phosphatase and RNAse activity of rat liver cell nuclei].[肝素、亚精胺和铍离子对大鼠肝细胞核磷酸酶和核糖核酸酶活性的影响]
Biull Eksp Biol Med. 1979 Jun;87(6):542-4.
6
Thyroid hormone increases type I adenosine 3', 5'-monophosphate-dependent protein kinase and casein kinase activities in rat liver cytosol: analysis of protein kinases by polyacrylamide disc gel electrophoresis.甲状腺激素增加大鼠肝细胞溶胶中I型腺苷3'，5'-单磷酸依赖性蛋白激酶和酪蛋白激酶活性：通过聚丙烯酰胺圆盘凝胶电泳分析蛋白激酶。
Endocrinology. 1983 Apr;112(4):1427-33. doi: 10.1210/endo-112-4-1427.
7
Ribofuranosyl-benzimidazole derivatives as inhibitors of casein kinase-2 and casein kinase-1.
Eur J Biochem. 1990 Jan 12;187(1):89-94. doi: 10.1111/j.1432-1033.1990.tb15280.x.
8
Casein kinase II is a major protein phosphorylating activity in the nuclei of Xenopus laevis oocytes.酪蛋白激酶II是非洲爪蟾卵母细胞核中一种主要的蛋白质磷酸化活性物质。
Biochem Int. 1987 Apr;14(4):707-17.
9
Phosphorylation of fibrinogen by casein kinase 2.酪蛋白激酶2对纤维蛋白原的磷酸化作用。
Biochem J. 1986 Mar 15;234(3):523-6. doi: 10.1042/bj2340523.
10
Cyclic AMP-independent casein/glycogen synthase kinases from pig polymorphonuclear leucocytes.来自猪多形核白细胞的不依赖环磷酸腺苷的酪蛋白/糖原合酶激酶
Biochem J. 1981 Mar 1;193(3):829-37. doi: 10.1042/bj1930829.
本文引用的文献
1
Isolation of pure and unaltered liver nuclei morphology and biochemical composition.分离出形态和生化组成纯净且未改变的肝细胞核。
Exp Cell Res. 1956 Aug;11(2):317-21. doi: 10.1016/0014-4827(56)90107-0.
2
Nuclear-protein changes in the cell cycle: interphase studies with regenerating rat liver.细胞周期中的核蛋白变化：对再生大鼠肝脏的间期研究
Biochem Soc Trans. 1980 Dec;8(6):759-66. doi: 10.1042/bst0080759.
3
Apparent two phase beryllium labelling of hepatic cell nuclei isolated after intravenous administration of beryllium compounds to rats. A possible explanation.给大鼠静脉注射铍化合物后分离出的肝细胞核出现明显的两相铍标记。一种可能的解释。
Arch Toxicol. 1980 May;45(1):75-80. doi: 10.1007/BF00303298.
4
Phosphorylation of glycogen synthase by cyclic AMP-independent casein kinase-2 from rabbit skeletal muscle.兔骨骼肌中不依赖环磷酸腺苷的酪蛋白激酶-2对糖原合酶的磷酸化作用。
J Biol Chem. 1982 Mar 25;257(6):3236-42.
5
Enzyme induction in rat liver: the effects of Be2+ in vivo.大鼠肝脏中的酶诱导：体内铍离子的影响。
Biosci Rep. 1981 Mar;1(3):217-22. doi: 10.1007/BF01114907.
6
The toxicity of beryllium.
Lab Invest. 1966 Jan;15(1 Pt 1):176-80.
7
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
8
Uptake, distribution and binding of beryllium to organelles of the rat liver cell.铍在大鼠肝细胞细胞器中的摄取、分布及结合
Biochem J. 1968 Feb;106(4):811-20. doi: 10.1042/bj1060811.
9
Effects of beryllium on deoxyribonucleic acid-synthesizing enzymes in regenerating rat liver.铍对再生大鼠肝脏中脱氧核糖核酸合成酶的影响。
Biochem J. 1970 Dec;120(3):623-34. doi: 10.1042/bj1200623.
10
A simple procedure for the purification of rat liver phosvitin kinase.
Biochim Biophys Acta. 1970 Sep 16;212(3):515-7. doi: 10.1016/0005-2744(70)90261-5.
Zotero 插件