Klebe R J, Mancuso M G
Somatic Cell Genet. 1982 Nov;8(6):723-30. doi: 10.1007/BF01543014.
Dimethyl sulfoxide (DMSO) has many biological effects, which include enhancement of polyethylene glycol (PEG) -mediated cell fusion, induction of cell differentiation in erythroleukemia and other cell systems, and cryoprotection of cells from freezing damage. In this study, compounds which induce erythroleukemia cell differentiation were tested for their ability to enhance PEG-mediated cell fusion. It was found that many compounds which induce erythroleukemia cell differentiation also promote cell membrane fusion as well as protect cells against freezing damage. Hence, many inducers of erythroleukemia cell differentiation have direct and similar effects on cell membranes. This study also demonstrates previously unrecognized effects of cryoprotective agents and cell fusogens on the differentiated state of cultured cells.
二甲基亚砜（DMSO）具有多种生物学效应，包括增强聚乙二醇（PEG）介导的细胞融合、诱导红白血病及其他细胞系统中的细胞分化，以及保护细胞免受冷冻损伤。在本研究中，对诱导红白血病细胞分化的化合物进行了增强PEG介导的细胞融合能力的测试。结果发现，许多诱导红白血病细胞分化的化合物也能促进细胞膜融合，并保护细胞免受冷冻损伤。因此，许多红白血病细胞分化诱导剂对细胞膜具有直接且相似的作用。本研究还证明了冷冻保护剂和细胞融合剂对培养细胞分化状态的此前未被认识到的作用。
