Zink M C, Johnson J A, Prescot J F, Pascoe P J
J Reprod Fertil Suppl. 1982;32:491-6.
The in-vitro interaction of Corynebacterium equi and foal alveolar macrophages was examined qualitatively and quantitatively using cells collected by sequential bronchoalveolar lavage at 2-week intervals from birth until 14 weeks of age. Total and differential counts were performed on the recovered cells. Macrophages were identified using the non-specific esterase strain. Cultures of the alveolar macrophages were challenged with C. equi suspensions and the process and extent of ingestion was examined by light and electron microscopy. Few macrophages were recovered from the lungs of foals less than 20 days old. The numbers increased with age in a biphasic pattern. The majority of the recovered cells were alveolar macrophages. Foal alveolar macrophages were capable of significant ingestion of C. equi only in the presence of immune serum. The ingestion of C. equi by foal alveolar macrophages was confirmed by electron microscopy.
使用从出生到14周龄每隔2周通过连续支气管肺泡灌洗收集的细胞，对马棒状杆菌与马驹肺泡巨噬细胞的体外相互作用进行了定性和定量研究。对回收的细胞进行了总数和分类计数。使用非特异性酯酶染色鉴定巨噬细胞。用马棒状杆菌悬液攻击肺泡巨噬细胞培养物，并通过光学显微镜和电子显微镜检查摄取过程和程度。在小于20日龄的马驹肺中回收的巨噬细胞很少。数量随年龄呈双相增加。回收的细胞大多数是肺泡巨噬细胞。马驹肺泡巨噬细胞仅在免疫血清存在下才能大量摄取马棒状杆菌。通过电子显微镜证实了马驹肺泡巨噬细胞对马棒状杆菌的摄取。
