Ishii S, Yamawaki S, Sasaki T, Usui M, Ubayama Y, Minaimi A, Yagi T, Isu K, Kobayashi M
Int Orthop. 1982;6(4):215-23. doi: 10.1007/BF00267142.
Thirty cases of human osteosarcoma were transplanted subcutaneously in BALB/c nude mice. Twenty-four tumours survived in animals and showed the histopathology of the original tumours. Growth of transplantable tumours was closely related to the amount of osteoid tissue and tumours with predominant osteoid tissue showed slow growth. Tumours whose osteoid-forming activity tended to diminish or disappear were likely to fail early during serial transplantation. Two osteosarcoma (SU and ISHI) were established into permanent transplantable strains and were maintained in nude mice for more than three years. These two strains showed striking differences in their growth capacity; SU grew rapidly and frequently formed pulmonary tumours after tail vein injection of tumour cells. Histologically, ISHI osteosarcoma cells continue to form osteoid tissue, whereas SU cells showed anaplastic changes and lost osteoid-forming activity. Alkaline phosphatase and Lactate Dehydrogenase (LDH) activities of tumour tissue were lower in ISHI than SU, and significant elevation of the relative value of Fraction III of LDH isozyme has been demonstrated in SU, and Fraction IV in ISHI. On this basis, the authors discuss the relation between the amount of osteoid formation or LDH isozyme patterns and the malignant potential of human osteosarcoma.
将30例人类骨肉瘤皮下移植到BALB/c裸鼠体内。24个肿瘤在动物体内存活，并呈现出原发肿瘤的组织病理学特征。可移植肿瘤的生长与类骨组织的量密切相关，以类骨组织为主的肿瘤生长缓慢。类骨形成活性趋于降低或消失的肿瘤在连续传代移植过程中早期可能失败。两种骨肉瘤（SU和ISHI）建立了永久可移植株，并在裸鼠体内维持了三年以上。这两种株系在生长能力上表现出显著差异；SU生长迅速，尾静脉注射肿瘤细胞后常形成肺肿瘤。组织学上，ISHI骨肉瘤细胞继续形成类骨组织，而SU细胞则表现出间变改变并失去类骨形成活性。ISHI肿瘤组织的碱性磷酸酶和乳酸脱氢酶（LDH）活性低于SU，并且在SU中已证实LDH同工酶Ⅲ组分的相对值显著升高，而在ISHI中Ⅳ组分升高。在此基础上，作者讨论了类骨形成量或LDH同工酶模式与人类骨肉瘤恶性潜能之间的关系。
