Schwartz M, Spirman N
Proc Natl Acad Sci U S A. 1982 Oct;79(19):6080-3. doi: 10.1073/pnas.79.19.6080.
The involvement of gangliosides in processes related to nerve regeneration and sprouting has been demonstrated recently. The type of interaction by which gangliosides may influence neuronal sprouting was investigated in the present work. Affinity-purified rabbit anti-GM1 antibodies were found to block the sprouting from dorsal root ganglia (DRG) of chicken embryo induced by nerve growth factor (NGF). Only a moderately inhibitory effect was produced by antibodies directed to GM2, suggesting a specific interaction between NGF and GM1. In spite of the low inhibitory effect of anti-GM2 antibodies on sprouting, their binding to dissociated DRG cells was found to be higher than that of anti-GM1 antibodies. These results emphasize the high resolution of the system and suggest that the interaction of NGF with GM1 is involved in the process of sprouting.
神经节苷脂参与神经再生和发芽相关过程的现象最近已得到证实。本研究探讨了神经节苷脂可能影响神经元发芽的相互作用类型。发现亲和纯化的兔抗GM1抗体可阻断神经生长因子（NGF）诱导的鸡胚背根神经节（DRG）发芽。针对GM2的抗体仅产生中度抑制作用，提示NGF与GM1之间存在特异性相互作用。尽管抗GM2抗体对发芽的抑制作用较弱，但发现它们与解离的DRG细胞的结合高于抗GM1抗体。这些结果强调了该系统的高分辨率，并提示NGF与GM1的相互作用参与了发芽过程。
